Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry
نویسندگان
چکیده
منابع مشابه
Characterisation of phosvitin phosphopeptides using MALDI-TOF mass spectrometry.
Putative phosphopeptides produced from enzyme hydrolysis of phosvitin were identified and characterised using MALDI-TOF/MS. Phosvitin was heat-pretreated and then hydrolysed using pepsin, thermolysin, and trypsin at their optimal pH and temperature conditions with or without partial dephosphorylation. Pepsin and thermolysin were not effective in producing phosphopeptides, but trypsin hydrolysis...
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ژورنال
عنوان ژورنال: Journal of Mass Spectrometry
سال: 2008
ISSN: 1076-5174,1096-9888
DOI: 10.1002/jms.1342